Review



primary normal adult human dermal fibroblast cell line from skin  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Thermo Fisher primary normal adult human dermal fibroblast cell line from skin
    Correlation between GST activity and fluorescence intensity in various cancer cell lines. ( A ) Seven cancer cell lines (MKN45, HuH-28, HGC27, DLD1, CCK81, M7609, and NUGC-4) were incubated with DNAT-Me at 10 μM for 10 min and observed under fluorescence microscopy with excitation at 488 nm. Fluorescence intensity per cell in each cell line was quantitated using software provided by the manufacturer, as described in Materials and Methods. A primary normal adult human dermal <t>fibroblast</t> line (HDFa) was used as a negative control. ( B ) The cytosolic fraction was extracted from each cell line, and GST activity was measured using 1-chloro-2,4- dinitrobenzene and glutathione as substrates.
    Primary Normal Adult Human Dermal Fibroblast Cell Line From Skin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+normal+adult+human+dermal+fibroblast+cell+line+from+skin/pmc05529364-169-8-20?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    primary normal adult human dermal fibroblast cell line from skin - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Molecular imaging of aberrant crypt foci in the human colon targeting glutathione S-transferase P1-1"

    Article Title: Molecular imaging of aberrant crypt foci in the human colon targeting glutathione S-transferase P1-1

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-06857-x

    Correlation between GST activity and fluorescence intensity in various cancer cell lines. ( A ) Seven cancer cell lines (MKN45, HuH-28, HGC27, DLD1, CCK81, M7609, and NUGC-4) were incubated with DNAT-Me at 10 μM for 10 min and observed under fluorescence microscopy with excitation at 488 nm. Fluorescence intensity per cell in each cell line was quantitated using software provided by the manufacturer, as described in Materials and Methods. A primary normal adult human dermal fibroblast line (HDFa) was used as a negative control. ( B ) The cytosolic fraction was extracted from each cell line, and GST activity was measured using 1-chloro-2,4- dinitrobenzene and glutathione as substrates.
    Figure Legend Snippet: Correlation between GST activity and fluorescence intensity in various cancer cell lines. ( A ) Seven cancer cell lines (MKN45, HuH-28, HGC27, DLD1, CCK81, M7609, and NUGC-4) were incubated with DNAT-Me at 10 μM for 10 min and observed under fluorescence microscopy with excitation at 488 nm. Fluorescence intensity per cell in each cell line was quantitated using software provided by the manufacturer, as described in Materials and Methods. A primary normal adult human dermal fibroblast line (HDFa) was used as a negative control. ( B ) The cytosolic fraction was extracted from each cell line, and GST activity was measured using 1-chloro-2,4- dinitrobenzene and glutathione as substrates.

    Techniques Used: Activity Assay, Fluorescence, Incubation, Microscopy, Software, Negative Control



    Similar Products

    90
    Thermo Fisher primary normal adult human dermal fibroblast cell line from skin
    Correlation between GST activity and fluorescence intensity in various cancer cell lines. ( A ) Seven cancer cell lines (MKN45, HuH-28, HGC27, DLD1, CCK81, M7609, and NUGC-4) were incubated with DNAT-Me at 10 μM for 10 min and observed under fluorescence microscopy with excitation at 488 nm. Fluorescence intensity per cell in each cell line was quantitated using software provided by the manufacturer, as described in Materials and Methods. A primary normal adult human dermal <t>fibroblast</t> line (HDFa) was used as a negative control. ( B ) The cytosolic fraction was extracted from each cell line, and GST activity was measured using 1-chloro-2,4- dinitrobenzene and glutathione as substrates.
    Primary Normal Adult Human Dermal Fibroblast Cell Line From Skin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+normal+adult+human+dermal+fibroblast+cell+line+from+skin/pmc05529364-169-8-20?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    primary normal adult human dermal fibroblast cell line from skin - by Bioz Stars, 2026-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    Correlation between GST activity and fluorescence intensity in various cancer cell lines. ( A ) Seven cancer cell lines (MKN45, HuH-28, HGC27, DLD1, CCK81, M7609, and NUGC-4) were incubated with DNAT-Me at 10 μM for 10 min and observed under fluorescence microscopy with excitation at 488 nm. Fluorescence intensity per cell in each cell line was quantitated using software provided by the manufacturer, as described in Materials and Methods. A primary normal adult human dermal fibroblast line (HDFa) was used as a negative control. ( B ) The cytosolic fraction was extracted from each cell line, and GST activity was measured using 1-chloro-2,4- dinitrobenzene and glutathione as substrates.

    Journal: Scientific Reports

    Article Title: Molecular imaging of aberrant crypt foci in the human colon targeting glutathione S-transferase P1-1

    doi: 10.1038/s41598-017-06857-x

    Figure Lengend Snippet: Correlation between GST activity and fluorescence intensity in various cancer cell lines. ( A ) Seven cancer cell lines (MKN45, HuH-28, HGC27, DLD1, CCK81, M7609, and NUGC-4) were incubated with DNAT-Me at 10 μM for 10 min and observed under fluorescence microscopy with excitation at 488 nm. Fluorescence intensity per cell in each cell line was quantitated using software provided by the manufacturer, as described in Materials and Methods. A primary normal adult human dermal fibroblast line (HDFa) was used as a negative control. ( B ) The cytosolic fraction was extracted from each cell line, and GST activity was measured using 1-chloro-2,4- dinitrobenzene and glutathione as substrates.

    Article Snippet: Adult human dermal fibroblasts (HDFa), as a primary normal adult human dermal fibroblast cell line from skin, were purchased from Thermo Fisher Scientific Inc. (Wilmington, DE).

    Techniques: Activity Assay, Fluorescence, Incubation, Microscopy, Software, Negative Control