primary normal adult human dermal fibroblast cell line from skin (Thermo Fisher)
Structured Review

Primary Normal Adult Human Dermal Fibroblast Cell Line From Skin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+normal+adult+human+dermal+fibroblast+cell+line+from+skin/pmc05529364-169-8-20?v=Thermo+Fisher
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Molecular imaging of aberrant crypt foci in the human colon targeting glutathione S-transferase P1-1"
Article Title: Molecular imaging of aberrant crypt foci in the human colon targeting glutathione S-transferase P1-1
Journal: Scientific Reports
doi: 10.1038/s41598-017-06857-x
Figure Legend Snippet: Correlation between GST activity and fluorescence intensity in various cancer cell lines. ( A ) Seven cancer cell lines (MKN45, HuH-28, HGC27, DLD1, CCK81, M7609, and NUGC-4) were incubated with DNAT-Me at 10 μM for 10 min and observed under fluorescence microscopy with excitation at 488 nm. Fluorescence intensity per cell in each cell line was quantitated using software provided by the manufacturer, as described in Materials and Methods. A primary normal adult human dermal fibroblast line (HDFa) was used as a negative control. ( B ) The cytosolic fraction was extracted from each cell line, and GST activity was measured using 1-chloro-2,4- dinitrobenzene and glutathione as substrates.
Techniques Used: Activity Assay, Fluorescence, Incubation, Microscopy, Software, Negative Control